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Brevet : EP1257638 - ISOLATS BACTERIENS DU GENRE KLEBSIELLA ET GENE D'ISOMALTULOSE SYNTHASE ISOLE A ...
Titre
ISOLATS BACTERIENS DU GENRE KLEBSIELLA ET GENE D'ISOMALTULOSE SYNTHASE ISOLE A PARTIR DE CES DERNIERS
N° et date de publication de la demande
EP1257638 - 20/11/2002
Type de la demande
A1
N° et date de dépôt
EP00906861.0 - 15/02/2000
N° et date de priorité
PCT/SG0000023 - 15/02/2000
Classification CIB
A01H 1/00 ; C12N 9/90 ; C12N 1/21 ; C12N 15/00 ; C12N 15/09 ; C12P 19/12 ; C12P 19/24 ; C12R 1/22 ; C12R 1/91
Classification CPC
C12N 9/90 ; C12P 19/12 ; C12P 19/24 ; C12Y 504/99011 ; C12R2001/22 ; C12N 1/205 ; C12N 9/90 ; C12Y 504/99011 ; C12P 19/24 ; C12P 19/12 ; C12N 1/205 ; C12R2001/22
Famille de brevets
DE60035801T2 ; JP2003523203A ; EP1257638A1 ; ATE368736T1 ; BR0017119A ; AU2000228416B2 ; CN1434861A ; US7235712B1 ; AU2841600A ; WO0160993A1
Abrégé
Two strains of a novel bacterial species, Klebsiella singaporensis LX3 and LX21, are claimed. A nucleotide sequence (kis) encoding a novel form of isomaltulose synthase, KIS, is also claimed. Also claimed is a method for production of isomaltulose in a plant, which method comprises introducing into a cell of such plant a nucleic acid sequence which encodes an enzyme which converts sucrose into isomaltulose in a manner which allows said cell to express said nucleic acid sequence, as well as a functional cloning method of isolating nucleotide sequence encoding the KIS protein comprising the steps of (a) preparing a gene bank from a donor organism that contains a DNA sequence coding for an isomaltulose biosynthesis activity in a suitable host organism, (b) screening the clones of interest from the gene bank by their enhanced reducing sugar content, and (c) isolating the clones which contain a DNA coding for a protein with isomaltulose biosynthesis activity.
INTERVENANTS
Déposant
INST OF MOLECULAR AGROBIOLOGY (INSTITUTE OF MOLECULAR AGROBIOLOGY) - SG
Titulaire
Agency for Science, Technology an
Inventeur
ZHANG LIAN HUI (ZHANG, LIAN, HUI) - SG
LI XIANZHEN (LI, XIANZHEN) - SG
ZHANG DAOHAI (ZHANG, DAOHAI) - SG
Mandataire
CABINET REGIMBEAU - 20 RUE DE CHAZELLES - 75847 PARIS CEDEX 17 - FR - N° Siren : 784661357
STATUT EN FRANCE : Dossier déchu définitivement
Délivrance
01/08/2007
Remise de traduction du brevet
04/01/2008 (BOPI 2008-01)
Date de constatation de déchéance
-
30/10/2009 (BOPI 2009-48)
Dernière annuité payée
08/02/2008
Quantième- N° de l'annuité payée
9
